abstract：:Although much explored, oral tolerance for treatment of autoimmune diseases still awaits the establishment of novel and effective vectors. We investigated whether the tolerogenic CTA1(R7K)-COL-DD fusion protein can be expressed in edible plants, to induce oral tolerance and protect against arthritis. The fusion protei...

journal_title：Plant biotechnology journal

authors： Hansson C,Schön K,Kalbina I,Strid Å,Andersson S,Bokarewa MI,Lycke NY

更新日期：2016-04-01 00:00:00

abstract：:Salinity and drought are major environmental factors limiting the growth and productivity of alfalfa worldwide as this economically important legume forage is sensitive to these kinds of abiotic stress. In this study, transgenic alfalfa lines expressing both tonoplast NXH and H(+)-PPase genes, ZxNHX and ZxVP1-1 from t...

journal_title：Plant biotechnology journal

authors： Bao AK,Du BQ,Touil L,Kang P,Wang QL,Wang SM

更新日期：2016-03-01 00:00:00

abstract：:Single-stranded oligonucleotides (ssODNs) can be used to direct the exchange of a single nucleotide or the repair of a single base within the coding region of a gene in a process that is known, generically, as gene editing. These molecules are composed of either all DNA residues or a mixture of RNA and DNA bases and u...

journal_title：Plant biotechnology journal

authors： Rivera-Torres N,Kmiec EB

更新日期：2016-02-01 00:00:00

abstract：:Differences in gene sequences, many of which are single nucleotide polymorphisms, underlie some of the most important traits in plants. With humanity facing significant challenges to increase global agricultural productivity, there is an urgent need to accelerate the development of these traits in plants. oligonucleot...

journal_title：Plant biotechnology journal

authors： Sauer NJ,Mozoruk J,Miller RB,Warburg ZJ,Walker KA,Beetham PR,Schöpke CR,Gocal GF

更新日期：2016-02-01 00:00:00

abstract：:Drought tolerance of the wheat cultivar Bobwhite was previously enhanced by transformation with a construct containing the wheat DREB3 gene driven by the stress-inducible maize Rab17 promoter. Progeny of a single T2 transgenic line were used as pollinators in crosses with four elite bread wheat cultivars from Western ...

journal_title：Plant biotechnology journal

authors： Shavrukov Y,Baho M,Lopato S,Langridge P

更新日期：2016-01-01 00:00:00

abstract：:Production of novel transgenic floricultural crops with altered petal properties requires transgenes that confer a useful trait and petal-specific promoters. Several promoters have been shown to control transgenes in petals. However, all suffer from inherent drawbacks such as low petal specificity and restricted activ...

journal_title：Plant biotechnology journal

authors： Azuma M,Morimoto R,Hirose M,Morita Y,Hoshino A,Iida S,Oshima Y,Mitsuda N,Ohme-Takagi M,Shiratake K

更新日期：2016-01-01 00:00:00

abstract：:Abscisic acid (ABA) regulates plant adaptive responses to various environmental stresses, while L-ascorbic acid (AsA) that is also named vitamin C is an important antioxidant and involves in plant stress tolerance and the immune system in domestic animals. Transgenic tobacco (Nicotiana tabacum L.) and stylo [Stylosant...

journal_title：Plant biotechnology journal

authors： Bao G,Zhuo C,Qian C,Xiao T,Guo Z,Lu S

更新日期：2016-01-01 00:00:00

abstract：:Plant cell cultures constitute eco-friendly biotechnological platforms for the production of plant secondary metabolites with pharmacological activities, as well as a suitable system for extending our knowledge of secondary metabolism. Despite the high added value of taxol and the importance of taxanes as anticancer c...

journal_title：Plant biotechnology journal

authors： Ramírez-Estrada K,Altabella T,Onrubia M,Moyano E,Notredame C,Osuna L,Vanden Bossche R,Goossens A,Cusido RM,Palazon J

更新日期：2016-01-01 00:00:00

abstract：:The 1980s and 1990s saw a major expansion of biotechnology into new areas of science including genomics and recombinant technologies. This was coupled to the widespread emergence of academics into the commercial sector as they were encouraged to spin out companies or commercialize their intellectual property. There we...

journal_title：Plant biotechnology journal

authors： Paul MJ,Thangaraj H,Ma JK

更新日期：2015-10-01 00:00:00

abstract：:Although plant biotechnology has been widely investigated for the production of clinical-grade monoclonal antibodies, no antibody products derived from transgenic plants have yet been approved by pharmaceutical regulators for clinical testing. In the Pharma-Planta project, the HIV-neutralizing human monoclonal antibod...

journal_title：Plant biotechnology journal

authors： Ma JK,Drossard J,Lewis D,Altmann F,Boyle J,Christou P,Cole T,Dale P,van Dolleweerd CJ,Isitt V,Katinger D,Lobedan M,Mertens H,Paul MJ,Rademacher T,Sack M,Hundleby PA,Stiegler G,Stoger E,Twyman RM,Vcelar B,Fischer

更新日期：2015-10-01 00:00:00

abstract：:Despite progress in the prevention and treatment of infectious diseases, they continue to present a major threat to public health. The frequency of emerging and reemerging infections and the risk of bioterrorism warrant significant efforts towards the development of prophylactic and therapeutic countermeasures. Vaccin...

journal_title：Plant biotechnology journal

authors： Streatfield SJ,Kushnir N,Yusibov V

更新日期：2015-10-01 00:00:00

abstract：:One goal of modern agriculture is the improvement of plant drought tolerance and water-use efficiency (WUE). Although stomatal density has been linked to WUE, the causal molecular mechanisms and engineered alternations of this relationship are not yet fully understood. Moreover, YODA (YDA), which is a MAPKK kinase gen...

journal_title：Plant biotechnology journal

authors： Meng LS,Yao SQ

更新日期：2015-09-01 00:00:00

abstract：:There is an urgent need to provide effective anti-HIV microbicides to resource-poor areas worldwide. Some of the most promising microbicide candidates are biotherapeutics targeting viral entry. To provide biotherapeutics to poorer areas, it is vital to reduce the cost. Here, we report the production of biologically ac...

journal_title：Plant biotechnology journal

authors： O'Keefe BR,Murad AM,Vianna GR,Ramessar K,Saucedo CJ,Wilson J,Buckheit KW,da Cunha NB,Araújo AC,Lacorte CC,Madeira L,McMahon JB,Rech EL

更新日期：2015-09-01 00:00:00

abstract：:A precursor of miR156 (MsmiR156d) was cloned and overexpressed in alfalfa (Medicago sativa L.) as a means to enhance alfalfa biomass yield. Of the five predicted SPL genes encoded by the alfalfa genome, three (SPL6, SPL12 and SPL13) contain miR156 cleavage sites and their expression was down-regulated in transgenic al...

journal_title：Plant biotechnology journal

authors： Aung B,Gruber MY,Amyot L,Omari K,Bertrand A,Hannoufa A

更新日期：2015-08-01 00:00:00

abstract：:Aphids produce gel saliva during feeding which forms a sheath around the stylet as it penetrates through the apoplast. The sheath is required for the sustained ingestion of phloem sap from sieve elements and is thought to form when the structural sheath protein (SHP) is cross-linked by intermolecular disulphide bridge...

journal_title：Plant biotechnology journal

authors： Abdellatef E,Will T,Koch A,Imani J,Vilcinskas A,Kogel KH

更新日期：2015-08-01 00:00:00

abstract：:The identification of genetic markers linked to genes of agronomic importance is a major aim of crop research and breeding programmes. Here, we identify markers for Yr15, a major disease resistance gene for wheat yellow rust, using a segregating F2 population. After phenotyping, we implemented RNA sequencing (RNA-Seq)...

journal_title：Plant biotechnology journal

authors： Ramirez-Gonzalez RH,Segovia V,Bird N,Fenwick P,Holdgate S,Berry S,Jack P,Caccamo M,Uauy C

更新日期：2015-06-01 00:00:00

abstract：:The intensification of agriculture and the development of synthetic insecticides enabled worldwide grain production to more than double in the last third of the 20th century. However, the heavy dependence and, in some cases, overuse of insecticides has been responsible for negative environmental and ecological impacts...

journal_title：Plant biotechnology journal

authors： Catarino R,Ceddia G,Areal FJ,Park J

更新日期：2015-06-01 00:00:00

abstract：:Tomato is one of the most cultivated vegetables in the world and an important ingredient of the human diet. Tomato breeders and growers face a continuous challenge of combining high quantity (production volume) with high quality (appearance, taste and perception for the consumers, processing quality for the processing...

journal_title：Plant biotechnology journal

authors： Czerednik A,Busscher M,Angenent GC,de Maagd RA

更新日期：2015-02-01 00:00:00

abstract：:Despite the declarations and collective measures taken to eradicate hunger at World Food Summits, food security remains one of the biggest issues that we are faced with. The current scenario could worsen due to the alarming increase in world population, further compounded by adverse climatic conditions, such as increa...

journal_title：Plant biotechnology journal

authors： Singh J,Pandey P,James D,Chandrasekhar K,Achary VM,Kaul T,Tripathy BC,Reddy MK

更新日期：2014-12-01 00:00:00

abstract：:In the past few years, the usefulness of transient expression assays has continuously increased for the characterization of unknown gene function and metabolic pathways. In grapevine (Vitis vinifera L.), one of the most economically important fruit crops in the world, recent systematic sequencing projects produced man...

journal_title：Plant biotechnology journal

authors： Jelly NS,Valat L,Walter B,Maillot P

更新日期：2014-12-01 00:00:00

abstract：:Lignocellulosic feedstocks can be converted to biofuels, which can conceivably replace a large fraction of fossil fuels currently used for transformation. However, lignin, a prominent constituent of secondary cell walls, is an impediment to the conversion of cell walls to fuel: the recalcitrance problem. Biomass pretr...

journal_title：Plant biotechnology journal

authors： Poovaiah CR,Nageswara-Rao M,Soneji JR,Baxter HL,Stewart CN Jr

更新日期：2014-12-01 00:00:00

abstract：:MicroRNAs (miRNAs) are a class of regulatory small RNAs (sRNAs) that down-regulate target genes through mRNA cleavage or translational inhibition. miRNA is known to play an important role in the root development and environmental responses in both the Arabidopsis and rice. However, little information is available to f...

journal_title：Plant biotechnology journal

authors： Kong X,Zhang M,Xu X,Li X,Li C,Ding Z

更新日期：2014-10-01 00:00:00

abstract：:S-adenosyl-L-methionine (SAM) synthetase is the key enzyme involved in the biosynthesis of SAM, which serves as a common precursor for polyamines (PAs) and ethylene. A SAM synthetase cDNA (SlSAMS1) was introduced into the tomato genome using the Agrobacterium tumefaciens transformation method. Transgenic plants overex...

journal_title：Plant biotechnology journal

authors： Gong B,Li X,VandenLangenberg KM,Wen D,Sun S,Wei M,Li Y,Yang F,Shi Q,Wang X

更新日期：2014-08-01 00:00:00

abstract：:Tef, Eragrostis tef (Zucc.) Trotter, is the most important cereal in Ethiopia. Tef is cultivated by more than five million small-scale farmers annually and constitutes the staple food for more than half of the population of 80 million. The crop is preferred by both farmers and consumers due to its beneficial traits as...

journal_title：Plant biotechnology journal

authors： Girma D,Assefa K,Chanyalew S,Cannarozzi G,Kuhlemeier C,Tadele Z

更新日期：2014-06-01 00:00:00

abstract：:Willows (Salix spp.) are important biomass crops due to their ability to grow rapidly with low fertilizer inputs and ease of cultivation in short-rotation coppice cycles. They are relatively undomesticated and highly diverse, but functional testing to identify useful allelic variation is time-consuming in trees and tr...

journal_title：Plant biotechnology journal

authors： Salmon J,Ward SP,Hanley SJ,Leyser O,Karp A

更新日期：2014-05-01 00:00:00

abstract：:Although conifers are of immense ecological and economic value, bioengineering of their chloroplasts remains undeveloped. Understanding the chloroplast genomic organization of conifers can facilitate their bioengineering. Members of the conifer II clade (or cupressophytes) are highly diverse in both morphologic featur...

journal_title：Plant biotechnology journal

authors： Wu CS,Chaw SM

更新日期：2014-04-01 00:00:00

abstract：:Next-generation sequencing technologies provide opportunities to understand the genetic basis of phenotypic differences, such as abiotic stress response, even in the closely related cultivars via identification of large number of DNA polymorphisms. We performed whole-genome resequencing of three rice cultivars with co...

journal_title：Plant biotechnology journal

authors： Jain M,Moharana KC,Shankar R,Kumari R,Garg R

更新日期：2014-02-01 00:00:00

abstract：:Grain size, number and starch content are important determinants of grain yield and quality. One of the most important biological processes that determine these components is the carbon partitioning during the early grain filling, which requires the function of cell wall invertase. Here, we showed the constitutive exp...

journal_title：Plant biotechnology journal

authors： Li B,Liu H,Zhang Y,Kang T,Zhang L,Tong J,Xiao L,Zhang H

更新日期：2013-12-01 00:00:00

abstract：:The root system is fundamental for plant development, is crucial for overall plant growth and is recently being recognized as the key for future crop productivity improvement. A major determinant of root system architecture is the initiation of lateral roots. While knowledge of the genetic and molecular mechanisms reg...

journal_title：Plant biotechnology journal

authors： Jansen L,Hollunder J,Roberts I,Forestan C,Fonteyne P,Van Quickenborne C,Zhen RG,McKersie B,Parizot B,Beeckman T

更新日期：2013-12-01 00:00:00

abstract：:Recent developments of tools for targeted genome modification have led to new concepts in how multiple traits can be combined. Targeted genome modification is based on the use of nucleases with tailor-made specificities to introduce a DNA double-strand break (DSB) at specific target loci. A re-engineered meganuclease ...

journal_title：Plant biotechnology journal

authors： D'Halluin K,Vanderstraeten C,Van Hulle J,Rosolowska J,Van Den Brande I,Pennewaert A,D'Hont K,Bossut M,Jantz D,Ruiter R,Broadhvest J

更新日期：2013-10-01 00:00:00

abstract：:Nanobodies® (VHHs) provide powerful tools in therapeutic and biotechnological applications. Nevertheless, for some applications, bivalent antibodies perform much better, and for this, an Fc chain can be fused to the VHH domain, resulting in a bivalent homodimeric VHH-Fc complex. However, the production of bivalent ant...

journal_title：Plant biotechnology journal

authors： De Buck S,Nolf J,De Meyer T,Virdi V,De Wilde K,Van Lerberge E,Van Droogenbroeck B,Depicker A

更新日期：2013-10-01 00:00:00

abstract：:Measurement of gene expression can provide important clues about gene function and molecular basis of developmental processes. Here, we have analysed the chickpea transcriptome in vegetative and flower tissues by exploiting the potential of high-throughput sequencing to measure gene expression. We mapped more than 295...

journal_title：Plant biotechnology journal

authors： Singh VK,Garg R,Jain M

更新日期：2013-08-01 00:00:00

abstract：:Sumoylation is a posttranslational regulatory process in higher eukaryotes modifying substrate proteins through conjugation of small ubiquitin-related modifiers (SUMOs). Sumoylation modulates protein stability, subcellular localization and activity; thus, it regulates most cellular functions including response to envi...

journal_title：Plant biotechnology journal

authors： Li Z,Hu Q,Zhou M,Vandenbrink J,Li D,Menchyk N,Reighard S,Norris A,Liu H,Sun D,Luo H

更新日期：2013-05-01 00:00:00

abstract：:Developing new strategies for crop plants to respond to drought is crucial for their innovative breeding. The down-regulation of nuclear cap-binding proteins in Arabidopsis renders plants drought tolerant. The CBP80 gene in the potato cultivar Desiree was silenced using artificial microRNAs. Transgenic plants displaye...

journal_title：Plant biotechnology journal

authors： Pieczynski M,Marczewski W,Hennig J,Dolata J,Bielewicz D,Piontek P,Wyrzykowska A,Krusiewicz D,Strzelczyk-Zyta D,Konopka-Postupolska D,Krzeslowska M,Jarmolowski A,Szweykowska-Kulinska Z

更新日期：2013-05-01 00:00:00

abstract：:The activation of phospholipase Dα1 (PLDα1) produces lipid messenger phosphatidic acid and promotes stomatal closure in Arabidopsis. To explore the use of the PLDα1-mediated signalling towards decreasing water loss in crop plants, we introduced Arabidopsis PLDα1 under the control of a guard cell-specific promoter AtKa...

journal_title：Plant biotechnology journal

authors： Lu S,Bahn SC,Qu G,Qin H,Hong Y,Xu Q,Zhou Y,Hong Y,Wang X

更新日期：2013-04-01 00:00:00

abstract：:Globally, wheat is the most widely grown crop and one of the three most important crops for human and livestock feed. However, the complex nature of the wheat genome has, until recently, resulted in a lack of single nucleotide polymorphism (SNP)-based molecular markers of practical use to wheat breeders. Recently, lar...

journal_title：Plant biotechnology journal

authors： Allen AM,Barker GL,Wilkinson P,Burridge A,Winfield M,Coghill J,Uauy C,Griffiths S,Jack P,Berry S,Werner P,Melichar JP,McDougall J,Gwilliam R,Robinson P,Edwards KJ

更新日期：2013-04-01 00:00:00

abstract：:Ascorbate is a powerful antioxidant in plants, and its levels are an important quality criteria in commercial species. Factors influencing these levels include environmental variations, particularly light, and the genetic control of its biosynthesis, recycling and degradation. One of the genes involved in the recyclin...

journal_title：Plant biotechnology journal

authors： Gest N,Garchery C,Gautier H,Jiménez A,Stevens R

更新日期：2013-04-01 00:00:00

abstract：:Antioxidants are protective molecules that neutralize reactive oxygen species and prevent oxidative damage to cellular components such as membranes, proteins and nucleic acids, therefore reducing the rate of cell death and hence the effects of ageing and ageing-related diseases. The fortification of food with antioxid...

journal_title：Plant biotechnology journal

authors： Zhu C,Sanahuja G,Yuan D,Farré G,Arjó G,Berman J,Zorrilla-López U,Banakar R,Bai C,Pérez-Massot E,Bassie L,Capell T,Christou P

更新日期：2013-02-01 00:00:00

abstract：:Which factors limit metabolite accumulation in plant cells? Are theories on flux control effective at explaining the results? Many biotechnologists cling to the idea that every pathway has a rate limiting enzyme and target such enzymes first in order to modulate fluxes. This often translates into large effects on meta...

journal_title：Plant biotechnology journal

authors： Morandini P

更新日期：2013-02-01 00:00:00

abstract：:Drought conditions are among the most serious challenges to crop production worldwide. Here, we report the results of field evaluations of transgenic rice plants overexpressing OsNAC5, under the control of either the root-specific (RCc3) or constitutive (GOS2) promoters. Field evaluations over three growing seasons re...

journal_title：Plant biotechnology journal

authors： Jeong JS,Kim YS,Redillas MC,Jang G,Jung H,Bang SW,Choi YD,Ha SH,Reuzeau C,Kim JK

更新日期：2013-01-01 00:00:00